Quick detection of Chlamydiales and Chlamydiaceae employing polymerase chain reaction

The study covered two simple and rapid PCR techniques on fresh field specimens of pneumonia, conjunctivitis, diarrhea, abortion, metritis, pooled visceral organs as well as stored chlamydiae infected lyophilized yolk sac membranes for fast detection of different members of Chlamydiales and Chlamydiaceae. The field samples belonged to different species of livestock, wild/zoo mammals and birds. The molecular diagnosis of chlamydial infections assumes significance as chlamydiae being intracellular bacteria and their isolation still confront. Overall, 453 samples comprising of 363 fresh and 90 lyophilized were processed for detection of chlamydiae through isolation, DNA extraction and later employing PCR tests. The two PCR tests carried provided superb sensitivity as well as easy recognition of chlamydial infections as compared to labour intensive isolation procedures and indirect micro-immunofluorescence test.