1Department of Veterinary Microbiology College of Veterinary and Animal Sciences, Pantnagar -263145 (U.A.).
2Department of Animal Biotechnology College of Veterinary and Animal Sciences, Pantnagar -263145 (U.A.).
*Corresponding Author: 51A, Vaibhav, Suncity Vistar, Bareilly (UP).
Proteinous molecules of Salmonella have been found to be the major virulence factors, viz., enterotoxin and cytotoxins. This paper report protein profiles of some important Salmonella serovars. The protein concentration in cefotaxime extract (CE) and precipitated dialyzed preparation (PDP) of Salmonella Weltevreden were 5.1 and 9.3 mg/ml, respectively. The PDPs of Salmonella Typhimurium, Salmonella Bareilly and Salmonella Gallinarum contained 6.1, 2.4 and 3.2 mg/ml, respectively. The SDS-PAGE revealed a total of 24 and 15 bands in CE and PDP of Salmonella Weltevreden, respectively. The PDPs of S. Gallinarum, S. Bareilly and S. Typhimurium yielded 16, 6 and 13 bands, respectively. On gel filtration through Sephacryl S-200HR, CE and PDP of Salmonella Weltevreden was fractioned into 6 peaks (protein ratio of 1:8:1:16:3:4) and 5 peaks (protein ratio 1:4:8:3:6 approximately), respectively. The PDPs of S. Gallinarum, S. Typhimurium and S. Bareilly yielded 6, 4 and 3 peaks containg protein in the ratio of 7:2:7:2:1:4, 1:2:3:5 and 3:4:1, respectively.
