The serosurveillance of infectious bovine rhinotracheitis in breeding cow bulls and buffalo bulls of different farms of India was carried out. The serum samples of bulls collected/received from different farms were screened for BHV1 antibodies by micro-serum neutralization test (SNT), a sensitive and reliable test recommended by OIE and accepted internationally in the trade of livestock. A total of 225 serum samples (189 cow bulls and 36 buffalo bulls) were screened for prevalence of BHV1 antibodies. A total of 75 (39.68%) serum samples of cow bulls and 21 (58.33%) of buffalo bulls respectively were found positive by micro-SNT. Overall 96 bulls (42.66%) were found positive for BHV-1 antibodies. The bulls of Military Farm, Lucknow showed the highest percent of positivity (77.78) followed by Regional Diagnostic Laboratory, Jalandhar (75.0), Central Semen Station, Durg (64.29), Frozen Semen Production Centre, Chak Gajaria, Lucknow (50) and Regional Disease Diagnostic Laboratory, Pune (43.8). The bulls of 4 farms were found to be negative for the presence of antibodies to BHV-1.
