The sources of microbial contamination in organized poultry slaughter house were enumerated by collecting 138 swab samples from 23 different points of a slaughter house and processing plant. Amongst all points, slaughter house floor yielded maximum TVC (5.86±0.26 log CFU/cm2±SE) followed by evisceration room floor (5.79±0.25 log cfu/cm2±SE) while packaging material (2.11±0.1 log CFU/cm2±SE) and chilled water (2.74±0.19 log CFU/cm2±SE) yielded minimum TVC. The pathogenic and spoilage-causing organisms showed similar trend with maximum contamination on the floors. The average bacterial count (log CFU/cm2±SE) of various organisms viz.