Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases
  • Year: 2011
  • Volume: 32
  • Issue: 1and2

Growth and cytopahogenecity of a field isolate of fowlpox virus in BGM-70 cell line

  • Author:
  • R.K. Joshi, Namita Joshi1
  • Total Page Count: 3
  • Page Number: 19 to 21

1Head, Department of Veterinary Public Health.

College of Veterinary Science and A.H. N.D. University of Agriculture and Technology, Kumarganj - 224 229, Faizabad (U.P.)

*Corresponding author: Head, Department of Veterinary Microbiology; e. mail - rkjoshivet26@gmail.com

Online published on 9 October, 2012.

Abstract

A field isolate of Fowlpox virus was attempted to grow in BGM-70 cell line. The adaptation and multiplication of virus was checked by study of cytopathic effects (CPE), infectivity titers and ELISA titer for viral antigen. The cellular changes were visible after three blind passages and characteristic CPE were consistently seen from 8th passage onwards. The cellular changes included rounding, clumping of cell at 48 hrs post infection (PI), vacuolation, intracytoplasmic inclusion at 72 hrs PI, Bollinger bodies at 96 hrs PI and microplaques at 120 hrs PI. The presence of viral DNA in cell cytoplasm in acridine orange staining and perinuclear fluorescence in FAT again confirmed the multiplication of virus in BGM-70 cells. Further, a progressive increase in infectivity and antigen mass as ELISA titre of Fowlpox isolate was indicative of its adaptation in BGM 70 cells.