In vitro expression profile of cytokine genes in chicken spleenocyte induced with two lactobacillus species

Probiotics act as alternative to antibiotics and play important role in promoting the growth and health of broiler chicken In ourstudy, different species of Lactobacilli were identified based on Gram's staining, biochemical characterization, and amplification of 16S rRNA gene and sequence analysis. Mononuclear spleen cells isolated from chicken were co-cultured with live L. salivarius, L. ingluviei to assess the immuomodulatory potential based on cytokine genes expression profile using real-time PCR. Among the two strains, the new isolate L. ingluviei significantly upregulated the proinflammatory cytokines such as IL12, IL18, gamma interferon (IFNγ), IL1β and anti-inflammatory cytokines such as IL10, transforming growth factor (TGFβ4) in the invitro cultured spleen cells.