Polymerase chain reaction for amplification of IL-1β gene from peripheral blood mononuclear cells of cattle

Interleukin-1 (IL-1) plays its role in inflammation, fever and release of acute phase proteins by acting on major organs of the body. This study is planned to standardise polymerase chain reaction (PCR) for amplification of IL-1β gene from bovine peripheral blood mononuclear cells (PBMCs). Blood samples were collected from cattle in containers with 0.1% ethylenediaminetetraacetic acid anticoagulant. PBMCs were isolated by using commercially available Histopaque^®-1077. Extracted total ribose nucleic acid was utilised in reverse transcription to prepare complementary deoxyribose nucleic acid (cDNA). The resulting cDNA was employed in PCR assay. IL-1β-specific primer set along with positive control was commercially procured to amplify partial nucleotide sequence of IL--1β gene successfully. The resulting amplified IL-1β DNA product (420 bp) was run on 1% agarose gel electrophoresis and documented by using gel documentation system.