1Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Mhow-453 446, Indore, Madhya Pradesh, India
2Project Directorate on Foot-and-Mouth Disease (PD-FMD), Indian Veterinary Research Institute Campus, Mukteshwar, Kumaon-263 138, Nainital, Uttarakhand, India
*Corresponding author E-mail: asd_vet@yahoo.com, audarya.sachin@gmail.com
Online published on 12 August, 2016.
Monocyte chemoattractant protein-1 (MCP-1) plays its role in various pathological conditions including atherosclerosis. This study is planned to standardise polymerase chain reaction (PCR) for amplification of MCP-1 gene from bovine peripheral blood mononuclear cells (PBMCs). Blood samples were collected from cattle in containers with 0.1% ethylenediaminetetraacetic acid anticoagulant. PBMCs were isolated by using commercially available Histopaque®-1077. Extracted total ribose nucleic acid was utilised in reverse transcription to prepare complementary deoxyribose nucleic acid (cDNA). The resulting cDNA was employed in PCR assay. MCP-1-specific primer set along with positive control was commercially procured to amplify partial nucleotide sequence of MCP-1 gene successfully. The resulting amplified MCP-1 DNA product (209 bp) was run on 1% agarose gel electrophoresis and documented by using gel documentation system.
Cytokine, MCP-1, Cattle, PCR, PBMCs
