Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases
  • Year: 2017
  • Volume: 38
  • Issue: 1

Screening of Breeding Bulls of Assam for Infectious Bovine Rhinotracheitis (IBR) by Polymerase Chain Reaction (PCR)

1Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-781022, Assam, India

2Department of Microbiology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-781022, Assam, India

3Department of Microbiology, North Eastern Regional Disease Diagnostic Laboratory, Khanapara, Guwahati-781022, Assam, India

4Department of Microbiology, Lakhimpur College of Veterinary Science, Assam Agricultural University, Joyhing, Lakhimpur-787051, Assam, India

*Corresponding author E-mail: shineygeorge0@gmail.com

Online published on 27 July, 2017.

Abstract

The present study was undertaken to screen the semen of breeding bulls of Assam for Infectious bovine rhinotracheitis virus. The danger of bovine herpes virus-1 (BHV-1) getting transmitted during artificial insemination through semen of clinically or subclinically infected pedigreed bulls is well known. Routine screening of semen lots prior to insemination by conventional diagnostic methods is laborious and may not be even sensitive enough to detect a very low level of infection. Therefore, in the present study, semen samples from breeding bulls were screened by polymerase chain reaction (PCR) for the presence of BHV-1. All the 31 semen samples tested with PCR was found negative.

Keywords

Infectious bovine rhinotracheitis, Bovine herpes virus-1, Breeding bull, Semen, PCR