Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases
  • Year: 2021
  • Volume: 42
  • Issue: 2spl

Isolation and molecular confirmation of leptospires from dogs in Himachal Pradesh, India

  • Author:
  • Nirdeshika Chaudhary1, Prasenjit Dhar2,, Mandeep Sharma3, Ajay Katoch4, Subhash Verma2, T. Sabarinath5, Pratiksha Srivastava6, Aneesh Thakur7
  • Total Page Count: 7
  • Page Number: 141 to 147

1Veterinary Officer, Veterinary Hospital Bhattu, Department of Animal Husbandry, Himachal Pradesh, India

2Professor, Department of Veterinary Microbiology, DGCN COVAS, CSKHPKV, Palampur-176062, Himachal Pradesh, India

3Professor and Dean, Department of Veterinary Microbiology, DGCN COVAS, CSKHPKV, Palampur-176062, Himachal Pradesh, India

4Assistant Professor, Department of Veterinary Medicine, DGCN COVAS, CSKHPKV, Palampur-176062, Himachal Pradesh,India

5Senior Scientist, Clinical Bacteriology Laboratory, ICAR-Indian Veterinary Research Institute, Mukteshwar Campus, India

6Veterinary Officer, People's Medical College, Bhopal, Madhya Pradesh, India

7Assistant Professor, Department of Pharmacy, University of Copenhagen, Universitetsparken 2, 2100, Copenhagen, Denmark

*Corresponding author E-mail id: thakurprasen727779@gmail.com

Online Published on 24 March, 2022.

Abstract

Leptospirosis is a common but largely underreported zoonotic disease in India. It has been recognized as a re-emerging infectious disease causing deaths in dogs as well as humans. Leptospires have been isolated from both clinically ill and apparently healthy animals from different geographical regions but there is no information on the presence of leptospires in various clinical conditions of animals in the northern state of Himachal Pradesh, India. Here, using dark field microscopy, culture isolation and molecular biology-based tests, we confirm leptospirosis in three clinically affected dogs with chronic renal involvement. The study reports the first isolation and molecular confirmation of leptospires from clinically affected dogs in Himachal Pradesh, India.

Keywords

Leptospirosis, Dogs, Isolation, PCR, India