Molecular Detection of African Swine Fever Virus (ASFV) and Porcine Reproductive and Respiratory Syndrome virus 2 (PRRSV-2) in Sikkim, India Using Real-Time PCR Assays

African swine fever virus (ASFV) and Porcine reproductive and respiratory syndrome virus Type 2 (PRRSV-2) are listed as notifiable diseases by the World Organisation for Animal Health due to their pathogenicity and their abilityto affect the swine industry, leading to economic losses. This study aims to address the paucity ofregion-specific data, including demographic and spatial risk factors. Analyzing a total of 182 biological samples collected from pigs in the period of 2022-2025, this study employed the OIE-recommended qPCR procedure. ASFV was detectedin 88.46%, and its prevalence increased across years, while PRRSV-2cases were limited to 2022 and found to have an 11.54% positivity rate. Statistically significant associations were observed betweenvirus prevalence and factors such as age group and sampling year (p < 0.0001). These findings are significant for understanding the shifting dynamics of swine viral infections in the region and guiding targeted disease monitoring.