1Research Scholar, Rhizosphere Biology Laboratory, Department of Environmental Microbiology, School for Environmental Sciences, Babasaheb Bhimrao Ambedkar University (A Central University), Vidya Vihar Raibareli Road, Lucknow
2M. Sc., Rhizosphere Biology Laboratory, Department of Environmental Microbiology, School for Environmental Sciences, Babasaheb Bhimrao Ambedkar University (A Central University), Vidya Vihar Raibareli Road, Lucknow
3Associate Professor, Rhizosphere Biology Laboratory, Department of Environmental Microbiology, School for Environmental Sciences, Babasaheb Bhimrao Ambedkar University (A Central University), Vidya Vihar Raibareli Road, Lucknow
*Corresponding author: Dr Rajesh Kumar Associate Professor, Department of Environmental Microbiology; Babasaheb Bhimrao Ambedkar University (A Central University), Vidya Vihar Raebareli Road, Lucknow-226 025 Contact No: +91-9412090052 Email-ID:: Rajesh_skumar@yahoo.co.in; Rajesh_dem@bbau.ac.in
Online published on 13 June, 2016.
The rise of heavy metal pollution and antibiotic resistant bacteria in soil and water is a key challenge to worldwide public health. The aim of current study is assessment of microbial load and co-resistance (cadmium and antibiotics) of bacteria isolated from the water of hospital waste discharge and soil of industrial of Lucknow and Allahabad (U.P. India). Soil samples were collected from different location of Uttar Pradesh. The samples were analyzed for presence of microbial load by heterotrophic plate count (HPC) ranged from 160cfu/ml-400x10⁵cfu/ml. Isolated bacteria assessed for multiple antibiotics and heavy metal resistance. The study was carried out on the basis of maximum threshold limit for the cadmium by the isolates. MIC test of isolates was performed for detection of tolerance against cadmium metal. From 34 potent isolates only ten isolates (AIA2, AIA4, BBAU2, BBAU4, SIA2, BSS1, BSS2, BSS4, HWS2, and HWS1) were able to grow at high cadmium level. While, out of ten only two isolates BSS2 and BSS4 found to grow at maximum threshold limit at 1100ppm. In antibiotic resistance test all the isolates had resistant property against cpm and most of the heavy metal resistant isolates had high multiple antibiotic resistant (MAR) index value. High MAR values were seen in BSS2 and AIA4 isolates.
Heavy metal, Co-resistance, Pollution, Antibiotics, MAR (Multiple Antibiotic Resistance), Cadmium
