Antitoxic and Bioenhancing role of Kamdhenu ark (Cow urine distillate) on fertilitty rate of male mice (mus musculus) affected by cadmium Chloride Toxicity

The study illustrates the antitoxic and bioenhancing role of Kamdhenu Ark (cow's urine distillate) in fertility rate of male mice affected by cadmium chloride toxicity. Twenty-five sexually mature male mice Mus musculus weighing about 35±5 gm were divided into five groups of five each. Group I, served as control received balanced diet and tap water, while animals of other groups II, III, IV and V received i.p. a single dose of cadmium chloride (CdCl₂, 0.005 mg/0.01ml/35±5 gms b.wt.). Besides this, group III, IV and V received ZnSO₄ (100 ppm), Kamdhenu ark (100 ppm) and Kamdhenu Ark+ZnSO₄ respectively for 60 days through drinking water. After ten days of the above treatment the male mice were allowed to mate with normal females and the fertility indices were observed. The control animal exhibited 100% fertility rate and delivered their litters after 30±3 days, with 100% viability and lactation indices. While, animals exposed with CdCl₂ only showed 0% fertility rate. However, the animals exposed with CdCl₂+Kamdhenu Ark+ZnSO₄ showed 90% fertility rate with 100% viability and lactation indices. Besides this, the fertility index was also found to be 88% in group treated with CdCl₂+Kamdhenu Ark. Thus, these results indicate that Kamdhenu Ark work as an antitoxic against the cadmium chloride toxicity and it can be used as a bioenhancer of Zn.