The objective of this study was to estimate the seroprevalence in the selected groups of cattle population in India. Sera samples (n=300) collected from adult cattle were tested by absorbed-ELISA and agar gel immunodiffusion (AGID) test. An absorbed-ELISA procedure standardised in cattle sera identified 64 (21.3%) and AGID test identified 7 (2.5%) animals positive for paratuberculosis infection.
Absorbed-ELISA, cattle, Johne's disease, seroprevalence
