Antagonistic effects of kamdhenu ark and its bioenhancing role with zinc in liver of male mus musculus(p) against cadmium toxicity

Twenty-five sexually mature male mice Mus musculus weighing about 35±5 gms were divided into five groups of five each. Group I, served as control received balanced diet and tap water, while animals of other groups II, III, IV and V received i.p. a single dose of cadmium chloride (CdCl₂, 0.005 mg/0.01ml/35±5 gms b. wt.). Alongwith this, group III, IV and V received ZnSO₄ (100 ppm), Kamdhenu ark (K.A.) only and Kamdhenu ark + ZnSO₄ respectively for 60 days through drinking water and histopathological studies were observed in liver of Mus musculus. Liver exposed to single injection of CdCl₂ up to 60 days showed severe necrotic changes. While, animals exposed with a single injection of CdCl₂ and 100 ppm of Zn up to 60 days showed less cellular damage in hepatocytes. However, the animal exposed with a single injection of CdCl₂ and 100 ppm of kamdhenu ark up to 60 days showed recovery in hepatocytes characterized by normal cellular features with prominent nuclei. In connection this, similar effects were also noticed when the animals exposed to single injection of CdCl₂, Zn and kamdhenu ark up to 60 days revealing normalcy of hepatocytes with no cellular disintegration in comparison to CdCl₂ treated only. All these observations may suggests that KA has antagonistic effects against the cadmium toxicity in liver of Mus musculus and it also work as bioenhancer of Zn.