Estimation of allelic frequency of -Casein and -Lactoglobulin genes in Bos Indicus cattle breeds

A polymerase chain reaction-restriction fragment length polymorphism test was performed on DNA extracted from blood samples of Bos indicus cattle breeds to estimate allelic frequency of the bovine kappa casein and beta-lactoglobulin gene responsible for milk production traits. A 350 bp fragment of kappa casein and 247 bp fragment for beta-lactoglobulin were amplified and digested with Hinf I and Hae III restriction enzymes, respectively. Two types of alleles A and B and three types of genotypes AA, BB and AB for kappa casein and beta-lactoglobulin were observed. In present study, the allelic frequency for B allele was found double (0.662) than A allele (0.338) for beta-lactoglobulin. Similarly allelic frequency for A allele was three and half times more than B allele for kappa-casein in Bos indicus cattle population. These genotypes are useful markers for milk production traits on which bull can be evaluated and selected for future breeding programmes.