The role of paraprobiotics in breast cancer cell line (MCF-7): In vitro study

Bioactive active metabolites seem promising in understanding the molecular pathology of cancer and especially in developing targeted therapies. In addition, the anticancer activity of some possible probiotic bacteria is emphasized in many literatures. Especially Lactobacillus strains and beneficial microbial activities are noteworthy. However, the use of live microorganisms in immunocompromised patients poses a risk. For this reason, our study aimed to evaluate the cytotoxic effect of inanimate/paraprobiotics obtained from probiotic microorganisms on the MCF-7 cell line, which is a breast cancer cell. L. rhamnosus ATCC 53103 and L. reuteri DSM 17938 strains and the MCF-7 cell line were used in our study. The cytotoxicity of paraprobiotics was determined by the MTT method. According to the obtained data, it was observed that paraprobiotics had a concentration and time dependent proliferative effect on healthy fibroblast cell lines and a cytotoxic effect dependent on the same parameters on MCF-7 cell lines. While the search for new pharmabiotic active molecules continues today, the identification of bioactive paraprobiotics will enable their use especially in individuals with immune system diseases and will allow the shelf life of biotic products to be longer.