1Plant Protection Department Isfahan University of Technology, Isfahan, Iran
2Plant Protection Department Islamic Azad University, Khorasgan Branch, Isfahan, Iran
3Institute of Biological Sciences, University of Malaya, Kuala Lumpur, Malaysia
*E-mail: rohollah_rajabi@yahoo.com
Online published on 14 October, 2013.
The advantage of a combination treatment with two entomopathogenic fungi Beauveria bassiana and Lecanicillium muscarium was assessed in laboratory bioassays on different nymphal stages of Trialeurodes vaporariorum. Among the fungal isolates, two isolates of each fungus differing in virulence from the highly virulent against T. vaporariorum were chosen. The results showed that there was a significant difference in mortality between the 103 conidia/ml and 106 conidia/ml for both L. muscarium and B. bassiana. The mortality due to the combination were generally higher than that caused by each in 106 conidia/ml. on young nymphs. The pathogenicity in the combination treatments 106 conidia/ml of L. muscarium with B. bassiana, no synergistic effect was observed. The mortality was higher in 103 conidia/ml of L muscarium plus 103 conidia/ml of B. bassiana compared to 103 conidia/ml of B. bassiana alone while there was no significant difference between 103 conidia/ml of L. muscarium and 103 conidia/ml of L muscarium plus 103 conidia/ml of B. bassiana. From these experiments, beneficial effect was observed in using the two fungi together compared compared to the separate application of B. bassiana.
Entomopathogenic fungi, Beauveria bassiana, Lecanicillium muscarium, Trialeurodes vaporariorum, Combination treatment
