Rapd profiling of oxyopidae spiders

Random amplified polymorphic DNA(RAPD) Polymerase Chain Reaction (PCR) was used to characterize six spiders belonging to the family Oxyopidae. The aim was to achieve genetic marker to determine the genetic variations of these species. The species studied includes: Oxyopes lineatipes (C L Koch, 1847), O. javanus (Thorell, 1887), O. shweta (Tikader, 1970), O. sunandae (Tikader, 1970), Hamadruas sp. (Thorell, 1887) and Peucetia viridana (Stolickza, 1869) collected from Karnataka. RAPD-PCR was carried out by using three random primers which generated polymorphic fragments. Genetic polymorphism among these samples was recorded by Neighbour Joining (NJ) method. The results revealed that their genetic distance ranged from 0.6962–0.8523. Cluster analysis based on this revealed that the tree is divided into two clades. The results concluded that the RAPD-PCR is an effective, fast and simple alternative method for identifying these spiders.