Indian Journal of Entomology
Open Access
SCOPUSWeb of Science
  • Year: 2019
  • Volume: 81
  • Issue: 4

Evaluation of substrates for mass culturing of Metarhizium anisopliae

1Department of Entomology, College of Agriculture, Sardar Vallabhbhai Patel University of Agriculture and Technology (SVPUAT), Meerut, 250110

2Department of Microbiology, College of Basic Sciences and Humanities, Punjab Agricultural University (PAU), Ludhiana, 141004

*Email: siwach2363@gmail.com (corresponding author)

Abstract

Entomopathogenic fungi are well known biological control agents and Metarhizium anisopliae is one among them. This study evaluates some substrates for mass production of this fungus. Both solid and liquid media were evaluated and it was observed that maximum sporulation was obtained with Sabouraud dextrose broth (18.39 spores/ml), a liquid media followed by sorghum grains (11.68 spores/ml), while sugarcane bagasse medium gave the least (2.86 spores/ml).

Keywords

Metarhizium anisopliae, sporulation, substrates, media, solid, liquid, sorghum, rice, FYM, vermicompost, vegetable waste, sugarcane bagasse, NSK, Sabourad dextrose, potato dextrose