Indian Journal of Entomology
Open Access
SCOPUSWeb of Science
  • Year: 2023
  • Volume: 85
  • Issue: 1

DNA barcoding of major insect pests and their natural enemies from cucurbitaceous crops in northeast India

  • Author:
  • Arensungla Pongen*, G T Behere1, D M Firake1, Bhagawati Sharma1, T Rajesh
  • Total Page Count: 5
  • Published Online: May 17, 2023
  • Page Number: 150 to 154

1Division of Crop Protection, ICAR Research Complex for NEH Region, Umiam, 793103, Meghalaya, India

School of Crop Protection, College of Post Graduate Studies, (Central Agricultural University), Umiam, 793103, Meghalaya, India

*Email: arensungpongen@gmail.com (corresponding author)

Online Published on 17 May, 2023.

Abstract

Correct identification of insect pest is a prerequisite for any control measures, and DNA barcoding facilitates this. In this study, assigning of 28 specimens (insect pests and natural enemies) to known species using DNA barcode by sequencing partial cytochrome oxidase I (COI) gene of mitochondrial DNA has been accomplished. Quick identification of a non-indigenous species Bactrocera cilifera (Diptera: Tephritidae) in Meghalaya has been enabled and taxonomic ambiguity of Henosepillachna pusillanima (Coleoptera: Coccinellidae) resolved. Molecular identity of Malcus sp., Paridea sp. and Coridius sp. has been established with NCBI GenBank registrations.

Keywords

Cucurbits, Insect pests, Natural enemies, Species identification, COI gene, Sequencing, Non-indigenous, Ambiguity, DNA barcodes, Molecular identity