Evaluation of a 16s rRNA amplicon illumina sequencing method for examining the gut microbiome of Lipaphis erysimi (kalt)

Herbivore specialists adapt to feed on a specific group of host plants by evolving various mechanisms to respond to plant defenses. Gene enrichment in the metagenome, accompanied by functional identification, revealed an essential role of specific gut bacteria in the breakdown of plant cell walls, detoxification of plant phenolics, and synthesis of amino acids. The Lipaphis erysimi (Kalt) gut bacteriome is an essential aspect for herbivory adaptation and this study is aimed at the microbiome of the musatrd Aphid L. erysimi gut by targeting the V3-V4 hyper variable region of the 16S rRNA gene with the Illumina platform sequencing using adult L. erysimi was performed to understand the bacteriome variations. A total of 103883 reads were obtained revealing the gut of the L. erysimi was found to be dominated by the main symbiont Buchnera a Protobacteria.