Indian Journal of Entomology

This study characterizes microsporidian spores in the wasp Polistes sp. using light bright field microscopy and scanning electron microscopy (SEM). Initial observations with light microscopy revealed ovoid spores exhibiting brownian movement and high birefringence. SEM provided detailed insights, revealing heavy infestations in the gut lining and spores ovoid shape averaging 1.75 μm in length and 1.20 μm in width (N=5). Various life stages were observed: binary fission, merogony, sporogony, and germination. The SEM images also highlighted sophisticated infection mechanisms, including anchoring discs and polar tube extrusions. The resilience and transmission capabilities of mature spores were noted, along with active infection indicators such as germinated spores. These findings emphasize the importance of SEM in revealing the morphological characteristics of microsporidian spores and their interactions with Polistes wasp. The high-resolution images provided critical insights into spore adherence, proliferation, and developmental stages, enhancing the understanding of the ecological impacts and co-evolutionary dynamics of microsporidian infections in insect populations.