Indian Journal of Entomology
Open Access
SCOPUSWeb of Science
  • Year: 2025
  • Volume: 87
  • Issue: 3

Phylogenetic Probe of Wolbachia in Bemisia tabaci

  • Author:
  • Junmoni Gayon1, Dilip Kumar Saikia1, Palash Debnath2, Surajit Kalita3,*
  • Total Page Count: 4
  • Published Online: Dec 17, 2025
  • Page Number: 711 to 714

1Department of Entomology, Assam Agricultural University, Jorhat785013, Assam

2Department of Plant Pathology, Assam Agricultural University, Jorhat785013, Assam

3Directorate of Research (Agri), Assam Agricultural University, Jorhat785013, Assam

*Email: surjit_kalita@yahoo.com (corresponding author)

Online Published on 17 December, 2025.

Abstract

The isolated DNA of Wolbachia present in whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleurodidae) collected from six different agroclimatic zones of Assam, India were amplified through polymerase chain reaction (PCR) using Wolbachia specific primer. A band in the range of 518-528 bp proved the presence of Wolbachia in the samples, which was further confirmed through BLAST analysis using the NCBI database. A phylogenetic analysis of the DNA sequences showed two subgroups of Wolbachia in the collected sample of B. tabaci. The W2 cluster formed with four samples of Wolbachia showed 91% similarity with Wolbachia from Drosophila simulans.

Keywords

Bemisia tabaci, Endosymbiont, Wolbachia, Wsp Primer, BLAST, Phylogenetic Analysis