Indian Journal of Ecology
Web of Science
  • Year: 2018
  • Volume: 45
  • Issue: 1

Molecular diversity analysis based on microsatellite markers in pearlmillet hybrids [Pennisetum glaucum (L.) R. BR.] and their parental lines

  • Author:
  • Hemender, Shikha Yashveer1, V.P.S. Sangwan, Sushma Sharma*, V.S. Mor
  • Total Page Count: 6
  • Page Number: 146 to 151

1Department of Molecular Biology, Biotechnology & Bioinformatics, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125 004, India

Department of Seed Science & Technology, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125 004, India

*E-mail: tanwar.sst@rediffmail.com

Online published on 30 March, 2022.

Abstract

To assess the genetic diversity among three pearlmillet hybrids and their parents, 55 microsatellite markers were used in the present study. Out of 55 markers, 37 amplified producing 162 alleles. The number of amplified alleles among the genotypes ranged from 1–9 per locus with a mean value of 4.38 alleles per locus. The highest PIC value obtained was for PSMP 2263 (0.837) with a range of 0 to 0.837 with an average PIC of 0.598. UPGMA cluster analysis differentiated all the genotypes in 2 clusters containing all the three hybrids. One A-line namely ICMA 94555 and both the R-lines (HBL 11 and H77/833-2-202) remained ungrouped. The results have revealed presence of considerable amount of genetic diversity among the pearlmillet genotypes and indicated the ability of SSR markers in recognizing the molecular diversity.

Keywords

Pearlmillet hybrids, A-lines, R- lines, SSRs, Genetic diversity