Physical and antimicrobial properties of Cissus quadrangularis stem extract incorporated edible films

Cissus quadrangularis, is widely accepted natural medicine for bone setting in Indian Ayurveda. The GC-MS analysis of this plant stem extract also confirmed the presence of phyto chemicals such as octadrine, piperidinol, phytol in it. Edible films (EFs) at different concentration of corn starch, glycerol and C. quadrangularis stem extract using Box Behnken method under Response Surface methodology. Thickness of the prepared EFs varied between 79–136μm and the opacity of the prepared EFs in the range of 20.31–30.45%. Tensile strength (S) (0.98–5.49 MPa) of the EFs was decreased significantly with increase in glycerol content. Increase in plant extract concentration significantly decreased TS of the EF. Water vapor permeability (0.739 * 10⁻⁷g/m. s. pa -10.9 *10⁻⁷ g/m. s. pa) of the EFs significantly decreased with increased starch, glycerol and plant extract concentrations. EFs with 15% plant extract showed antimicrobial effect on E. coli bacteria. Optimized EF formulation contains 3.33, 20 and 15 per cent starch, glycerol and C. quadrangularis stem extract.