Assessment of Neem (Azadirachta Indica) and Lemongrass (Cymbopogon citratus) Extracts on Meloidogyne incognita

Several Phyto-nematicides are available but none of them is capable to control Meloidogyne species infestation efficiently. Thus, there is a great need for an eco-friendly, highly effective sustainable control measure for this pest. In this study, to manage the Meloidogyne infestation, leaf extract of Azadirachta indica and Cymbopogon citratus were tested. Organic compounds present in extracts were characterized by GCMS. In vitro bioassays were conducted by applying three concentrations over 100 individuals of the 2nd juvenile stage. The mortality status of the nematode population by counting the dead and live individuals after applying 100 μl of the extract was recorded at different time durations i.e., 24, 48 and 72 hr. Results indicated significant variability toward different concentrations and durations for both extracts. GC-MS profiles of C. citratus and A. indica revealed the presence of diverse types of compounds in varying quantities. In such a way active compounds present in A. indica and C. citratus will be screened out. These compound/s-based formulations will be available for Integrated pest management programs, especially in horticultural crops to minimize infestation of Meloidogyne incognita.