Insecticidal activity of Tetraclinis articulata L. essential oils on Tribolium castaneum (Herbst 1797) (Tenebrionidae: Coleoptera) adults

Experimental work consists in highlighting the insecticidal properties of Tetraclinis articulata L. essential oils extracted from leaves and dry grains on the adults of Tribolium castaneum. under laboratory conditions. The fumigation treatments using four doses of Tetraclinis articulata leaf and dry kernels essential oils showed an insecticidal effect on Tribolium castaneum adults. High significant difference between grains and dry leaves T. articulata essential oils was observed on T. castaneum mortality. Mortality of 36.66 to 60% of mortality observed after 48 h of the treatment with dry grains oils. This toxicity reaches 86.66 and 96.66% respectively after the 4^th and the 5^th day of exposure. Whereasfor dry leaves, the mortality was 36.66 to 53.33% after 48h of treatment and 83.33 and 100% mortality respectively on the 4^th and 5^th day of exposure. The respective LD₅₀ and DL₉₀ doses for dry grains oils were 10.67 and 15.99 μl; whereas, for dry leaves oils the corresponding values were 9.23 μl for the LD₅₀ and 12.55 μl for the DL₉₀. Moreover, the measurement of the shorter time mortality highlights TL₅₀ = 36.13 h and TL₉₀ = 102.42 h for dry grain and a TL₅₀ = 38.02 h and TL₉₀ = 108.44 h for treatment based on dry leaves. Contact and ingestion methods can be also investigated to determine the T. articulata essential oils activity on other stages of this insect.