Synergistic Effect of Prebiotic with Gut Isolated Probiotic Bacteria on Survival, Growth and Carcass Composition of Labeo rohita

Feeding trial (120 days) was conducted to evaluate the synergistic effects of dietary supplementation of prebiotic and probiotic bacteria (Lactobacillus plantarum FLB1), isolated from the intestine of Indian Major Carp (Labeo rohita) on the survival, growth and carcass composition of L. rohita fingerlings. Six experimental diets were formulated containing basal diet as control (T1) and prebiotics, i.e. β-glucan (BG) and Mannan oligosaccharide (MOS) were added in basal diet (T1) at 2 different levels (T2 @ 0.2% BG + 10⁸L. plantarum, T3 @ 0.5% BG + 10⁸L. plantarum , T4 @ 0.2% MOS+10⁸L. plantarum , T5 @ 0.5% MOS + 10⁸L. plantarum , and T6 @ 10⁸L. plantarum) and fed to the fish for a period of 120 days @ 5% body weight in two parts, daily. Hematological parameters were observed at 30, 60 and 120 days intervals while growth parameters at 30, 60, 90 and 120 days intervals. Total body length (cm) and fish body weight (g) were significantly higher in T3. Average hemoglobin content, total erythrocyte count, total leukocyte count, hematocrit and erythrocyte sedimentation rate of fishes fed with T3 diet were significantly improved. Mean corpuscular volume, corpuscular hemoglobin and corpuscular hemoglobin content revealed positive effect in pre-and pro-biotic fed fishes. There was significant improvement in the carcass composition in T3 in terms of highest flesh protein as compared to other treatments and control.