In vitro Bio-efficacy of Trichoderma Based Nano Formulation against Pyricularia oryzae and Bipolaris oryzae

Biological synthesis of nanoparticle is very popular for its eco-friendly and non-toxic and green nature. Plant, Bacteria, Fungi, Yeast are used in this method. Among them Fungi are now the preferred option for production of nanoparticles due to their many benefits over other microorganisms. Trichoderma based biocontrol is a viable option in this situation. The goal of this project was to biologically synthesise silver nanoparticles (AgNP) from Trichoderma asperellum and Trichoderma harzianum, two possible native biocontrol agents. Silver nitrate (1 mM and 2 mM) served as the precursor for AgNP formation. Fourier Transmission Infrared Scanning and a UV-Vis spectrophotometer were used to characterise the biosynthesized silver nanoparticle. A characteristic UV-Vis absorption peak was observed at 415–420 nm, confirming the presence of AgNPs. In vitro bioefficacy was done by food poison technique. Silver nanoparticles at several concentrations (10 ppm, 5 ppm, and 1 ppm) were tested for their antagonistic activity in vitro against Pyricularia oryzae and Bipolaris oryzae, two Paddy diseases.