International Journal of Economic Plants

  • Year: 2019
  • Volume: 6
  • Issue: 3

Molecular Detection and Phylogenetic Analysis of Cucumber Mosaic Virus Infecting Bhut Jolokia (Capsicum chinense Jacq.) of Assam

1Plant Virology Laboratory Department of Plant Pathology, Assam Agricultural University, Jorhat, Assam (785 013), India

2Division of Crop Improvement, Central Potato Research Institute, Shimla, H.P. (171 001), India

Abstract

Prevalence of Cucumber mosaic virus (CMV) in Bhut Jolokia (Capsicum chinense Jacq.) in Assam was confirmed by reverse transcription polymerase chain reaction (RT-PCR). The symptomatic expression of CMV infection onto Bhut Jolokia was confirmed as chlorotic yellow mosaic appearance of the leaves, smaller leaves, reduction of plant growth, mosaic and mottling of infected leaves, leaves showing shoestring or filliform symptom and leaf puckering based on RT-PCR. This is the first report on molecular characterization of CMV in Assam, India. The RT-PCR diagnosis revealed presence of CMV with a band length of 593bp which has been obtained from CMV infected samples. The amplified coat protein gene of CMV was cloned and sequenced. Phylogenetic analysis of coat Protein gene of CMV showed close relatedness with African isolates of sub group IB and other isolates of the world.

Keywords

Assam, Capsicum chinense, cucumber mosaic virus, phylogenetic tree, RT-PCR