Evaluation of Silver Retention in Different Organs of Zebrafish (Danio Rerio) Fed Diet Supplemented With Silver Nanoparticles

To examine the effect of dietary silver nanoparticles (Ag-NPs) on different organs of D. rerio as well as evaluating of its retention and toxicity under laboratory conditions. Four experimental diets were designed with a concentration of 1, 4, 6 and 8 mg.kg-1 (ppm) nanoparticles in the diet (named C1, C4, C6 and C8, respectively) and control group without any nanoparticles (C0). Fish were fed with dry feed for a period of 56 days. At the end of feeding trial, the fish were randomly selected for determining silver retention in different organs including liver, intestine, kidney and gill. Characterization of silver nanoparticles was determined by transmission electron microscopy (TEM) and UV-Vis spectrophotometer. Histological examination of tissues showed evidence of lesions or damage to tissues in treatments to silver nanoparticles. Examination of different organs revealed highest concentration of Ag-NPs in liver, gills and muscle in the ranking order. It can be concluded that supplementation of fish diet with Ag-NPs particles can induce sever histological lesions in Zebrafish tissues.