Optimization of bacteriocin production by Pediococcus acidilactici MPKI using response surface methodology

In the present study culture conditions for bacteriocin production by Pediococcus acidilactici MPK1 in modified MRS medium were optimized by response surface methodology. The response studied was bacteriocin production in terms of zone of inhibition against Staphylococcus aureus MTCC 737 by agar well diffusion method. The results were analyzed by statistical method. Analysis of variance (ANOYA) and Fischer'S F test showed value of F 10.06 and demonstrated the significance of the regression model. The regression equation obtained indicated R² value of 0.9006 and ensured a perfect adjustment of quadratic model to experimental data. In the study optimum bacteriocin production (inhibition zone 17.73 mm) with R7.RO% desirahilitv was found at 35°C. pH 7.0 and incuhation time 39.57 h of growth of bacteria.