FTIR study of Nickel and Mercury induced biochemical changes in the muscles tissues of Lates Calcarifer

Nickel and mercury are naturally occurring, highly toxic environmental pollutant. The goal of the present study was investigate the effects of nickel and mercury exposure on the biochemical contents of the muscles tissues of fish Lates calcarifer by using Fourier transform infrared (FT-IR) spectroscopy. FT-IR spectra reveal significant differences in absorbance intensities between the control. Nickel and mercury intoxicated tissues, reflecting an alteration on the major biochemical constituents, such as lipids, proteins and nucleic acids of the muscles tissues of Lates calcarifer. Further, it has been observed that mercury has highly altered the biochemical constituents than nickel. Therefore this result concluded that FT-IR spectroscopy can be a successful detection tool in toxicological research.