Effects of chlorpyrifos on enzymes as biomarkers of toxicity in Fresh water field crab Barytelphusa guerini

The fresh water field crab, Barytelphusa guerini is an important commercial crustacean being widely distributed along the Andhra Pradesh and some other parts of India. The crab is constantly exposed to pesticides, which are used extensively to control agricultural pests. Evaluation of toxic effect of chlorpyrifos on crab was carried out. Effect of 1/3^rd and 1/4^th LC₅₀ concentrations of chlorpyrifos was carried out in the sensitive organs such as nervous tissue, thoracic ganglia and eyestalk. Alterations in the Lactate dehydrogenase (LDH), Succinate dehydrogenase (SDH), Acid phosphatase (ACP) and alkaline phosphatase (ALP) was carried out. The enzyme LDH was elevated throughout the experimented period and the SDH, ACP and ALP activity was inhibited along the experimental period at two sub-lethal concentrations.