Aqueous two-phase poly (ethylene glycol) – Maltodextrin system for protein partitioning from shrimp waste: Influence of molecular weight and pH

The polysaccharide maltodextrin (Mdx) can provide a low cost alternative to substitute the fractioned dextran for the use with polyethylene glycol (PEG) in aqueous two-phase polymer -polymers systems. Aqueous two-phase system (ATPS) partitioning has been used to recover and concentrate proteins from Shrimp Waste and offers many advantages along with biomass removal. In this work we have studied the partitioning proteins in poly (ethylene glycol)(PEG)-maltodextrin (Mdx) aqueous two-phase systems. The influence of the polymers molecular weights, pH, and addition of sodium chloride were investigated. The protein partitioning decreases on increasing the molecular weight of PEG, whereas the partitioning increases with increase in pH and addition of sodium chloride of the system.