Purification and Characterization of chitinase from Thermophilic Staphylococcus sp.

The objective of the research was to study the purification and partial characterization of thermophilic chitinase from the newly isolated Staphylococcus sp. The enzyme was purified. The enzyme was of 66 kDa as was evident by native PAGE. The protein was showing activity at pH 7 and 9. The optimum temperature activity was 60°C. Influence of metal ions such as calcium chloride (CaCl₂), Zinc sulfate (ZnSO₄), Magnesium sulfate (MgSO₄), Manganese sulfate (MnSO₄) was observed. Substrate specificity of the enzyme was also studied. Phylogenetic tree of the producer organism was also done.