Department of Biotechnology, School of Life Sciences, Khandari Campus Dr. Bhim Rao Ambedkar University, Agra-282004, Uttar Pradesh, India
*Email: mailtomonikasaxena@gmail.com
Online published on 24 April, 2015.
Dye is a major pollutant in textile effluents. Biological methods are considered cheap and ecologically safe to remove such pollutants. Present study demonstrated 11 bacterial cultures that were isolated from Yamuna water and textile effluent (6 and 5 cultures respectively) as an important weapon to be used in biological system. Of these, 7 cultures were Gram negative cocci, 3 cultures were Gram negative bacilli and only one culture was Gram positive bacilli. All these cultures were showed decolorization capacity for Acid Green dye (9.3% to 97.1%). The most promising cultures (S.2.1 and S.3.2) for decolorization were from Yamuna water (>90%). The other efficient decolorizing cultures (S.4.2, S.4.3 and S.5.1) were from textile effluent (83.7% to 86.1%). Thin layer chromatography of cultures (S.2.1 and S.3.2) did not show any dye specific spot in comparison to dye sample suggested possibility of biodegradation.
Bacteria, pollutants, TLC, spectroscopy, biodegradation