1Department of Soil Science and Agricultural Chemistry, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
2Department of Soil Science and Agricultural Chemistry, Agricultural Collage and Research Institute, Killikulam, 625282, Tamil Nadu, India
3Department of Environmental Science, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
*Email: dbalatnau@gmail.com
Online published on 24 April, 2015.
Microorganisms are known for their ability to metabolize a variety of chemical compound, including aliphatic and aromatic hydrocarbon, fatty acids and other environmental pollutants. These capabilities make them useful for application as bioremediation agents. The main objective of the study is to recover chromium reducing bacterial isolates from Palar river basin, Vellore, Tamil Nadu, by using enrichment method. Among the isolates recovered, two (CTBI 1 & CTBI 2) of the isolates were selected based on their chromium reduction capacity. The Potential strains were identified through morphological characterization, biochemical characterization and 16S rRNA gene analysis. The sequence of CTBI 1 and CTBI 2 shows a sequence homology of 95.68% with Pseudomonas putida and 95.19% with Bacillus subtilis respectively. Further it is concluded that the present method provides a viable and environmental friendly metal remediation technique.
Pseudomonas putida, Bacillus subtilis, Phylogenetic analysis, Cr (VI) reduction
