International Journal of Environmental Sciences
Open Access
  • Year: 2016
  • Volume: 6
  • Issue: 6

Cypermethrin induced DNA damage in Labio Rohita assessed by comet assay

  • Author:
  • Mohini Gadhia1, Rakesh Prajapati2, Pankaj Gadhia3
  • Total Page Count: 4
  • Page Number: 1113 to 1116

1Department of Aquatic Biology, Veer Narmad South Gujarat University, Saurat

2Department of Zoology, H & HB Kotak Institute of Science, Rajkot

3S. N. Gene Laboratory and Research Centre, President Plaza-A Ring Road, Surat. pankajkgadhia@gmail.com

Online published on 14 November, 2017.

Abstract

Toxicants exposure on fish may induce genetic alterations that can be used as genotoxic markers. We evaluated DNA damage using a Single Cell Gel Electrophoresis assay (SCGE), applied on erythrocytes after exposure of Labeo rohita to sub-lethal (0.03 ppm), lethal (0.06 ppm) and acute lethal (0.10 ppm) concentrations of Cypermethrin. Results revealed a significant level of DNA damages at all concentrations tested. Damage index of comet assay in L. rohita’s erythrocytes showed significant differences between sublethal, lethal and acute lethal exposures.

Keywords

Comet assay, Labeo rohita, DNA damage, Cypermethrin. Erythrocytes