As technology has evolved available guidelines for normal-phase flash chromatography have become less relevant. Years of experience performing chromatography with disposable columns have been condensed into simple guidelines useful for translating TLC results into either isocratic- or gradient-flash chromatography. The described studies should provide researchers with a means of selecting adequate columns and guidelines to reduce the waste of solvents, silica, time, and money.
Silica gel, Flash chromatography
