During the in vitro assay of antagonism of Trichoderma atroviride and Trichoderma herzanium were screened against the five dominant soil fungi viz. Aspergillus niger, Colletotrichum falcatum, Curvularia lunata and Alternaria alternatawerestudied by percent growth of inhibition of Trichoderma atroviride result were shows the highest growth of inhibition was seen against the Alternaria alternata (66.66%) after that Curvularia lunata(62.82%) and Aspergillus flavus (59.61%) the lowest percent of growth inhibition was seen Colletotrichum falcatum (53.84)and Aspergillus niger (56.25%)and Trichoderma harzianumshows highest potency against the Curvularia lunata (68.57%),next to that Aspergillus flavus (63.46%) and Alternaria alternata (60.00%), the lowest inhibition of Aspergillus niger(52.08%) Colletotrichum falcatum(58.97%).