Eco-friendly Microbial Decolorization and Detoxification of Reactive dyes by Isolated Three Culture of Bacillus sp.

Complete decolorization of Reactive dyes i.e. Reactive yellow 15, Reactive yellow 145 and Reactive red 195 has been observed at concentration of 500 mg/l within minimum time of 4–18 hrs in static condition of growth by three different bacterial isolates namely, Fictibacillus gelatini, Bacillus subtilis subsp. inaquosorum and Bacillus subtilis subsp. subtilis isolated from soil and dry sludge, near textile dyeing industry. These selected isolates showed even higher decolorization in simple nutrient broth media than in optimized media having combination of maltose and yeast extract at 37°C and neutral pH. Either they showed similar results as obtained in nutrient broth or showed less decolorization with supplementation. This suggests that carbon and nitrogen sources acted as a catabolite repessor. Current study is environmental-friendly due to use of biological methods and without any hazardous end products, highly economical as microbes utilized dyes as carbon and nitrogen source for their effective growth apart from complete decolorization that is occurring very fast within hours.