1Department of Engineering Chemistry, A. U. College of Engineering (A), Visakhapatnam-530003, (AP), India
2Department of Basic sciences and Humanities, Centurion University of Technology and Management
Online published on 4 January, 2021.
A simple and sensitive extractive visible spectrophotometric method for the assay of Darunavir (DNV) in pure and pharmaceutical formulations is developed., The method is based on oxidizable centre of primary amine the of selected drug reacts with MBTH reagent in presence of Ce (IV) to give coloured species at absorption peak of λmax at 518 nm. Beer's law is obeyed in the concentration range 4-24 μg/ml, the Molar absorptivity and Sandell sensitivity are 1.8295 x105 and 1.3540 x10-3 μg cm-2 respectively. The method proposed gave reproducible results with the percentage recoveries in the formulations found to be 99.800 to 99.949. The proposed method is cheap, accurate and can be successfully applied for the determination of DNV.
Spectrophotometry, MBTH, Cerium (IV), Anti retroviral, Pharmaceutical formulations
