In-vitro study of scilla indica (an Indian Medicinal Plant): The effect of different pgrs on direct somatic embryogenesis

Leaf explants of Scilla indica were cultured in vitro on modified MS medium. These explants initiated somatic embryos, which provided a sterile source of secondary somatic embryos’ explants. The secondary explants responded similarly to various combinations of plant growth regulators. The number of somatic embryos initiated was increased by the addition of cytokinins like IBA. BAP, 2iPA or thidiazuron (TDZ) in combination with naphthalene acetic acid (NAA). 2iPA is the most effective cytokinin in combination with NAA for direct somatic embryogenesis and their subsequent growth in Scilla indica. Separate root induction medium was not required because roots are generated in the same shoot induction medium itself. The plantlets were successfully acclimatized in shade house.