1Department of Microbiology, ASC College, Jalna, India
2Department of Medical Microbiology, Greater Kailash Hospital, Indore, India
3Department of Biotechnology, MGM College, Aurangabad, India
4Deptepartment of Biotechnology, University of Houston, Clear Lake, USA
*Email: vrtembhurkar@gmail.com
Online published on 19 March, 2013.
Influence of few growth conditions and media components on alpha amylase production by Bacillus thuringiensis in lab scale batch process were determined. B. thuringiensis produced maximum amylase after 72hrs of incubation. The optimum temperature and pH for amylase production were found to be 31°C and 7, respectively. The optimized media composed of [g/l] 10.0g Rice flour; 5.0 Peptone; CaSO4 4.0; MgSO4 2.0; NaCl 1.0; FeSO4 0.5 and pH 7. The alpha amylase produced was working optimally at 60°C, pH 5, [S] 20 mg/mL, [E] 2.0 mL. Many of these parameters are in compliance with the insecticidal crystal protein production by B. thuringiensis. Therefore, the process can be used for simultaneous production of alpha amylase and bioinsecticide in a single fermentation cycle.
Bacillus thuringiensis, Alpha amylase, Insecticide Crystal proteins, growth
