Sequential optimization of xylanase production by an indigenously isolated Acinetobacter species using solid state fermentation

BThe present study aimed at sequential optimization of culture conditions for the enhanced production of xylanase from indigenously isolated Acinetobacter sp. by solid state fermentation. Among various agro-industrial residues utilized wheat bran was found to be the best. Various fermentation parameters for xylanase production were optimized using one factor at a time method. At the end of 72 hours, maximum enzyme activity (7.52 U/gds) was obtained using wheat bran with 75% moisture content. Xylanase titres were further improved to 27.68U/gds using L₉(3⁴) orthogonal array method. Thus, approximately a four-fold increase in xylanase activity was achieved suggesting that newly isolated strain is an efficient xylanase producer.