1Assistant Professor, Department of Forensic Medicine and Toxicology, Shimoga Institute of Medical Sciences, Rajiv Gandhi University of Health Sciences, Karnataka, India
2Associate Professor, Department of Forensic Medicine and Toxicology, Shimoga Institute of Medical Sciences, Rajiv Gandhi University of Health Sciences, Karnataka, India
3Undergraduate, Shimoga Institute of Medical Sciences, Rajiv Gandhi University of Health Sciences, Karnataka, India
*Corresponding author: Dr. Veeresh MR Associate Professor, Department of Forensic Medicine And Toxicology, Shimoga Institute of Medical Sciences, Sagar Road, Shivamogga, Karnataka-577201. Ph: 9448319300, Email: Drveereshmr@gmail.com
Online published on 17 August, 2016.
Majority of sexual assaults remain unreported either due to lack of awareness or social stigma, accused being a family member and to avoid traumatizing situation of investigation. In the absence of DNA profiling, demonstration of biological material most commonly epithelial cells acts as a definite evidence of penetration.
Study was taken up to detect a suitable method for detection of vaginal epithelial cells and compare between Hemotoxylin and Eosin (H&E), Papanicolaou (PAP), and Lugol's staining methods.
Vaginal smears were prepared by obtaining samples from female dead bodies subjected for autopsy by roll over method and were stained with 3 stains and examined under 100 High Power Fields (HPF) microscopically.
From the study we found that among the different stains studied PAP staining detected the maximum number of cells per HPF with average number of cells detected being relatively superior in the head region of the slide.
In the absence of DNA profiling demonstration of Vaginal epithelial cells by any of the staining techniques can be corroborative.
Vaginal epithelial cells, Staining technique, Detection rate, Menstrual Phase
