Sequencing Analysis of Interleukin-4 gene 590C>T in Patients with Type-2 Diabetes Mellitus

The regulation of immune system at multiple levels is related with IL-4. Diabetes is a global health problem and is the sixth leading cause of death in the world.

In this study, we analyze the mutational changes related with polymorphisms of IL-4-590 C>T gene among64 patients with type-2 diabetes melliyus (T2DM) and 26 healthy individuals as control group. Patients and controls were from Wasit province in Iraq.

The study design was case-control study.

The allele-specific PCR (ARMS-PCR) technique was used to detect the IL-4-590 C>T gene polymorphism (rs2243250). PCR products for IL-4-590 C>T and primers were prepared for sequencing analysis in order to detect mutations and alterations in IL-4-590 C>T gene of patients with T2DM.

After alignment of product amplification of IL-4 590C>T CC or CT genotypes, there are five transversion substitution mutations in patients with diabetes mellitus table 2. Transversion substitution mutations: thymine to adenine T>A in site 864, Thymine to guanine T>G in site 814, transversion thymine to guanine in site 859 and in site 860.

We conclude that different transversion mutations at IL-4 gene 590C>T are associated with type-2diabetes mellitus.