Application of Silver Oxide Nanoparticles as Antibiofilm and Detection of Beta Lactamase Gene in Escherichia Coli Isolated from Diabetic Mellitus Patients

Wound and urine samples of a total of 124 patients with diabetes admitted to the Erbil Teaching and Layla health center in 2017 were inspected, and 80 patients were identified with E.coli. The identification was performed through evaluation of colony appearance, morphological properties, biochemical methods, and the VITEK 2 system. In addition to these, biofilm formation properties were also evaluated using three different methods, namely the micro titer plate, Congo red, and air liquid. For antibiofilm, all micro titer-plate isolated samples were treated with 100μg/ml AgO nanoparticles and the measurements were made with ELISA reader at 630nm. VITEK 2 system was used to test the antibiotic susceptibility of the E.coli samples, using a total of 16 antibiotics. bla_SHV and bla_CMY genes series were amplified used in determination through PCR test. Identification reference/electrophoresis sequencing identifications revealed 100% match for all isolates of E.coli. Finally, silver nanoparticles were used as the antibacterial agent for the antibiofilm formation tests.